Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM.
Identifieur interne : 002570 ( Main/Exploration ); précédent : 002569; suivant : 002571Characterization of a variant virus from ascitic fluid of subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 mice persistently infected with murine coronavirus strain JHM.
Auteurs : Shigeru Kyuwa [Japon] ; Satoshi Takagaki ; Shutoku Matsuyama ; Fumihiro Taguchi ; Junzo Saegusa ; Yoichiroh Iwakura ; Yoh-Ichi Tagawa ; Yasuhiro YoshikawaSource :
- Viral immunology [ 1557-8976 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Hépatite virale animale (anatomopathologie), Hépatite virale animale (immunologie), Hépatite virale animale (virologie), Interféron gamma (déficit), Interféron gamma (génétique), Liquide d'ascite (virologie), Mutation ponctuelle, Protéines de l'enveloppe virale (génétique), Souris, Souris de lignée C57BL, Souris knockout, Sérite (anatomopathologie), Sérite (virologie), Variation génétique, Virulence, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (pathogénicité).
- MESH :
- anatomopathologie : Hépatite virale animale, Sérite.
- déficit : Interféron gamma.
- génétique : Glycoprotéines membranaires, Interféron gamma, Protéines de l'enveloppe virale, Virus de l'hépatite murine.
- immunologie : Hépatite virale animale.
- pathogénicité : Virus de l'hépatite murine.
- virologie : Hépatite virale animale, Liquide d'ascite, Sérite.
- Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Mutation ponctuelle, Souris, Souris de lignée C57BL, Souris knockout, Variation génétique, Virulence.
English descriptors
- KwdEn :
- Animals, Ascitic Fluid (virology), Female, Genetic Variation, Hepatitis, Viral, Animal (immunology), Hepatitis, Viral, Animal (pathology), Hepatitis, Viral, Animal (virology), Interferon-gamma (deficiency), Interferon-gamma (genetics), Membrane Glycoproteins (genetics), Mice, Mice, Inbred C57BL, Mice, Knockout, Murine hepatitis virus (genetics), Murine hepatitis virus (pathogenicity), Point Mutation, Serositis (pathology), Serositis (virology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Virulence.
- MESH :
- chemical , deficiency : Interferon-gamma.
- chemical , genetics : Interferon-gamma, Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Murine hepatitis virus.
- immunology : Hepatitis, Viral, Animal.
- pathogenicity : Murine hepatitis virus.
- pathology : Hepatitis, Viral, Animal, Serositis.
- virology : Ascitic Fluid, Hepatitis, Viral, Animal, Serositis.
- Animals, Female, Genetic Variation, Mice, Mice, Inbred C57BL, Mice, Knockout, Point Mutation, Spike Glycoprotein, Coronavirus, Virulence.
Abstract
Previously, we showed that intraperitoneal infection with murine coronavirus strain JHM (JHMV) established a persistent infection with subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 (B6-GKO) mice. Herein, we characterize a variant virus from B6-GKO mice persistently infected with JHMV. Viruses were isolated from ascites at 25 d post-infection and cloned by limiting dilution on DBT cells; one variant was named 25V16G. To compare pathogenicity in vivo, we inoculated 25V16G and JHMV intraperitoneally into 8- to 12-week-old B6-GKO mice. Whereas nearly all of the B6-GKO mice infected with JHMV survived over 14 d, all of those infected with 25V16G died by 9 d post-infection. Histopathological examination revealed that 25V16G induced acute fulminant hepatitis in B6-GKO mice, whereas JHMV caused severe but focal hepatitis. The virus titer of 25V16G in the liver was 50- and 250-fold higher than that of JHMV at 5 and 7 d post-infection, respectively. However, there was no significant difference in viral growth between 25V16G and JHMV in cell lines cultured in vitro. Nucleotide sequencing of the S gene of 25V16G and JHMV revealed a deletion of 29 amino acids encompassing S(511-539), which covers a major cytotoxic T lymphocyte (CTL) epitope in C57BL/6 mice, and two point mutations resulting in amino acid changes in the S protein of 25V16G. One explanation for the greater pathogenicity of 25V16G is that 25V16G escapes CTL-mediated protection in B6-GKO mice. This experimental model may be used to assess the role of IFN-gamma in viral persistence in vivo.
DOI: 10.1089/vim.2010.0008
PubMed: 20712488
Affiliations:
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Tokyo</orgName></affiliation></author><author><name sortKey="Takagaki, Satoshi" sort="Takagaki, Satoshi" uniqKey="Takagaki S" first="Satoshi" last="Takagaki">Satoshi Takagaki</name></author><author><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name></author><author><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name></author><author><name sortKey="Saegusa, Junzo" sort="Saegusa, Junzo" uniqKey="Saegusa J" first="Junzo" last="Saegusa">Junzo Saegusa</name></author><author><name sortKey="Iwakura, Yoichiroh" sort="Iwakura, Yoichiroh" uniqKey="Iwakura Y" first="Yoichiroh" last="Iwakura">Yoichiroh Iwakura</name></author><author><name sortKey="Tagawa, Yoh Ichi" sort="Tagawa, Yoh Ichi" uniqKey="Tagawa Y" first="Yoh-Ichi" last="Tagawa">Yoh-Ichi Tagawa</name></author><author><name sortKey="Yoshikawa, Yasuhiro" sort="Yoshikawa, 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Tagawa</name></author><author><name sortKey="Yoshikawa, Yasuhiro" sort="Yoshikawa, Yasuhiro" uniqKey="Yoshikawa Y" first="Yasuhiro" last="Yoshikawa">Yasuhiro Yoshikawa</name></author></analytic><series><title level="j">Viral immunology</title><idno type="eISSN">1557-8976</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Ascitic Fluid (virology)</term><term>Female</term><term>Genetic Variation</term><term>Hepatitis, Viral, Animal (immunology)</term><term>Hepatitis, Viral, Animal (pathology)</term><term>Hepatitis, Viral, Animal (virology)</term><term>Interferon-gamma (deficiency)</term><term>Interferon-gamma (genetics)</term><term>Membrane Glycoproteins (genetics)</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Murine hepatitis virus (genetics)</term><term>Murine hepatitis virus (pathogenicity)</term><term>Point Mutation</term><term>Serositis (pathology)</term><term>Serositis (virology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Virulence</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Hépatite virale animale (anatomopathologie)</term><term>Hépatite virale animale (immunologie)</term><term>Hépatite virale animale (virologie)</term><term>Interféron gamma (déficit)</term><term>Interféron gamma (génétique)</term><term>Liquide d'ascite (virologie)</term><term>Mutation ponctuelle</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris knockout</term><term>Sérite (anatomopathologie)</term><term>Sérite (virologie)</term><term>Variation 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Variation</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Mice, Knockout</term><term>Point Mutation</term><term>Spike Glycoprotein, Coronavirus</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Mutation ponctuelle</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Souris knockout</term><term>Variation génétique</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Previously, we showed that intraperitoneal infection with murine coronavirus strain JHM (JHMV) established a persistent infection with subacute granulomatous serositis in interferon-gamma-deficient C57BL/6 (B6-GKO) mice. Herein, we characterize a variant virus from B6-GKO mice persistently infected with JHMV. Viruses were isolated from ascites at 25 d post-infection and cloned by limiting dilution on DBT cells; one variant was named 25V16G. To compare pathogenicity in vivo, we inoculated 25V16G and JHMV intraperitoneally into 8- to 12-week-old B6-GKO mice. Whereas nearly all of the B6-GKO mice infected with JHMV survived over 14 d, all of those infected with 25V16G died by 9 d post-infection. Histopathological examination revealed that 25V16G induced acute fulminant hepatitis in B6-GKO mice, whereas JHMV caused severe but focal hepatitis. The virus titer of 25V16G in the liver was 50- and 250-fold higher than that of JHMV at 5 and 7 d post-infection, respectively. However, there was no significant difference in viral growth between 25V16G and JHMV in cell lines cultured in vitro. Nucleotide sequencing of the S gene of 25V16G and JHMV revealed a deletion of 29 amino acids encompassing S(511-539), which covers a major cytotoxic T lymphocyte (CTL) epitope in C57BL/6 mice, and two point mutations resulting in amino acid changes in the S protein of 25V16G. One explanation for the greater pathogenicity of 25V16G is that 25V16G escapes CTL-mediated protection in B6-GKO mice. This experimental model may be used to assess the role of IFN-gamma in viral persistence in vivo.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement><orgName><li>Université de Tokyo</li></orgName></list><tree><noCountry><name sortKey="Iwakura, Yoichiroh" sort="Iwakura, Yoichiroh" uniqKey="Iwakura Y" first="Yoichiroh" last="Iwakura">Yoichiroh Iwakura</name><name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name><name sortKey="Saegusa, Junzo" sort="Saegusa, Junzo" uniqKey="Saegusa J" first="Junzo" last="Saegusa">Junzo Saegusa</name><name sortKey="Tagawa, Yoh Ichi" sort="Tagawa, Yoh Ichi" uniqKey="Tagawa Y" first="Yoh-Ichi" last="Tagawa">Yoh-Ichi Tagawa</name><name sortKey="Taguchi, Fumihiro" sort="Taguchi, Fumihiro" uniqKey="Taguchi F" first="Fumihiro" last="Taguchi">Fumihiro Taguchi</name><name sortKey="Takagaki, Satoshi" sort="Takagaki, Satoshi" uniqKey="Takagaki S" first="Satoshi" last="Takagaki">Satoshi Takagaki</name><name sortKey="Yoshikawa, Yasuhiro" sort="Yoshikawa, Yasuhiro" uniqKey="Yoshikawa Y" first="Yasuhiro" last="Yoshikawa">Yasuhiro Yoshikawa</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Kyuwa, Shigeru" sort="Kyuwa, Shigeru" uniqKey="Kyuwa S" first="Shigeru" last="Kyuwa">Shigeru Kyuwa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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